Eluted samples were then desalted with using a Hitrap desalting column. in buffer B (10?mM Tris-HCl, 5?mM EDTA, 8?M urea, 5?mM DTT) and kept at space temperature for 1?h. Presence of GST tagged protein was verified by SDS-PAGE and western blot using GST antibodies. Protein purification was carried out using … Continue reading Eluted samples were then desalted with using a Hitrap desalting column